of this process is the ability to couple to the information processing systems of the host cell using synthetic systems with
various signal-response characteristics. Here, we present a synthetic gene system in Escherichia coli whose signal-response curve may be tuned from band detection (strongest response within a band of input concentrations) to
a switch-like sigmoidal response, simply by altering the temperature. This change from a band-detection response to a sigmoidal
response has not previously been implemented. The system allows investigation of a range of signal-response behaviours with
minimal effort: a single system, once inserted into the cells, provides a range of response curves without any genetic alterations
or replacement with other systems. By altering its output, the system may couple to other synthetic or natural genetic circuits,
and thus serve as a useful modular component. A mathematical model has also been developed which captures the essential qualitative
behaviours of the circuit.
- Content Type Journal Article
- DOI 10.1007/s11047-009-9167-3
- Authors
- Sangram Bagh, University of Toronto Mississauga Department of Chemical and Physical Sciences, Institute for Optical Sciences 3359 Mississauga Rd. N Mississauga ON L5L 1C6 Canada
- David R. McMillen, University of Toronto Mississauga Department of Chemical and Physical Sciences, Institute for Optical Sciences 3359 Mississauga Rd. N Mississauga ON L5L 1C6 Canada
- Journal Natural Computing
- Online ISSN 1572-9796
- Print ISSN 1567-7818